Fig. 5.

KD modulated DC function via the PI3K-AKT-FoxO1 axis in DCs. (A) Transcript levels of PI3K, AKT, and FoxO1 in DCs. (B) Total and phosphorylated levels of PI3K, AKT, and FoxO1 proteins of DCs. (C) Levels of nuclear FoxO1 protein of DCs. (D) Levels of cytoplasmic FoxO1 protein of DCs. (E) Immunofluorescence of FoxO1 (green) in DCs. BMDCs isolated from control mice or LF mice treated with or without KD (30 mg/kg). (F) ChIP assay exploring the physical binding between FoxO1 and PD-L1 promoter in DCs. IgG was used as the matched control. LPS-stimulated DCs were either exposed to KD (5 μg/mL) or not. Data expressed as mean ± SD (n = 6). *P < 0.05, **P < 0.01 relative to controls; ^#P < 0.05, ^##P < 0.01 relative to CCl₄ or LPS groups. n.s., not significant.