Fig. 6.

KD inhibited DC cross-priming of HSC responses via reduction of IL-12 release from DCs. (A) Transcript levels of α-SMA, TGF-β₁, and TIMP-1 in JS-1, as assessed by qPCR. (B) Extracellular secretion level of Col-I, TGF-β₁, and TIMP-1 in JS-1 tested by ELISA. (C-D) Protein levels of α-SMA in JS-1, as detected by WB and immunofluorescence. JS-1, with or without IL-12, was co-cultured with DCs isolated from normal or LF mice pre-treated with KD, si-IL-12, or KD+si-IL-12. Data expressed as mean ± SD (n = 6). ^**P < 0.01 relative to normal DCs group; ^##P < 0.01 relative to LF DCs group. n.s., not significant.