Figure 1.

(a) Diagram of the Schwann cells and MC3T3-E1 cell coculture model; (b) representative images showing ALP staining; (c) ALP activity was measured with an ALP assay kit, n = 6, p = 0.0108; l; (d) representative images showing Alizarin red staining; l; (e) real-time RT-PCR (qPCR) analyses. ^∗P < 0.05, Student's t-test. N = 4; (f–i) transcriptome differences between the coculture group and the control group in MC3T3-E1 cells; (f) the boxplots represent the distribution of expression levels; the ordinate is gene expression level; (g) volcano plot of differential gene expression analysis; the dotted blue line indicates the threshold of the differential genes selected; (h) cluster heatmap of differentially expressed genes; (i) the KEGG enrichment scatter plot. The size of the black spots represents the gene number; the gradual color represents the P value.