Table 1.
Gene | Primer sequence | Initial denaturation | # of cycles | Cycling conditions | Final extension | Product size (bp) | References | ||
---|---|---|---|---|---|---|---|---|---|
Denaturation | Annealing | Extension | |||||||
hilA | F: 5′ CGGAAGCTTATTTG CGCCATGCTGAGGTAG 3′ |
94°C, 5 m | 30 | 94°C, 1 m | 65°C, 1 m | 72°C, 1 m | 72°C, 10 m | 854 | [26] |
R: 5′ GCATGGATCCCCG CGGCGAGATTGTG 3′ | |||||||||
spiC | F: 5′ CCTGGATAATGAC TATTGAT 3′ |
94°C, 3 m | 30 | 94°C, 1 m | 55°C, 1 m | 72°C, 1 m | 72°C, 5 m | 309 | [21, 28] |
R: 5′ AGTTTATGGTG ATTGCGTAT 3′ | |||||||||
misL | F:5′ GTCGGCGAATGC CGCGAATA 3′ |
94°C, 3 m | 30 | 94°C, 1 m | 55°C, 1 m | 72°C, 1 m | 72°C, 5 m | 550 | [21, 28] |
R: 5′ GCGCTGTTAAC GCTAATAGT 3′ | |||||||||
orfL | F: 5′ GGAGTATCGAT AAAGATGTT 3′ |
94°C, 3 m | 30 | 94°C, 1 m | 55°C, 1 m | 72°C, 1 m | 72°C, 5 m | 350 | [21, 28] |
R: 5′ GCGCGTAACGTC AGAATCAA 3′ | |||||||||
pipD | F: 5′ CGGCGATTCATG ACTTTGAT 3′ |
94°C, 5 m | 34 | 94°C, 25°s | 56°C, 30°s | 72°C, 50°s | 72°C, 5 m | 400 | [21, 28] |
R: 5′ CGTTATCATTCG GATCGTAA 3′ | |||||||||
sopB | F: 5′ TCAGAAGRCGTC TAACCACTC 3′ |
94°C, 3 m | 30 | 94°C, 1 m | 55°C, 1 m | 72°C, 1 m | 72°C, 5 m | 517 | [27] |
R: 5′ TACCGTCCTCA TGCACACTC 3′ | |||||||||
stn | F: 5′TTGTGTCGCTATCA CTGGCAACC 3′ |
94°C, 5 m | 25 | 94°C, 1 m | 59°C, 1 m | 72°C, 1 m | 72°C, 10 m | 617 | [26] |
R: 5′ ATTCGTAACCCG CTCTCGTCC 3′ | |||||||||
fliC | F: 5′ CGGTGTTGCCCA GGTTGGTAAT 3′ |
94°C, 3 m | 30 | 94°C, 1 m | 55°C, 1 m | 72°C, 1.5 m | 72°C, 10 m | 620 | [26] |
R: 5′ ACTGGTAAAGAT GGCT 3′ | |||||||||
pefA | F: 5′ TGTTTCCGGGCT TGTGCT 3′ |
94°C, 5 m | 25 | 94°C, 55°s | 55°C, 55°s | 72°C, 55°s | 72°C, 10 m | 700 | [26] |
R: 5′ CAGGGCATTTGC TGATTCTTCC 3′ | |||||||||
spvA | F: 5′GTCAGACCCGT AAACAGT 3′ |
94°C, 5 m | 30 | 94°C, 30°s | 60°C, 30°s | 72°C, 1 m | 72°C, 5 m | 604 | [29] |
R: 5′ GCACGCAGAG TACCCGCA 3′ | |||||||||
spvB | F: 5′ ACGCCTCAGCG ATCCGCA 3′ |
94°C, 5 m | 30 | 94°C, 30 s | 60°C, 30°s | 72°C, 1 m | 72°C, 5 m | 1063 | [29] |
R: 5′ GTACAACATCT CCGAGTA 3′ | |||||||||
spvC | F: 5′ CGGAAATACCA TCAAATA 3′ |
94°C, 5 m | 30 | 93°C, 1 m | 42°C, 1 m | 72°C, 2 m | 72°C, 4°m | 669 | [26] |
R: 5′ CCCAAACCCAT ACTTACTCTG 3′ |