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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Nat Struct Mol Biol. 2021 Dec 30;29(1):40–46. doi: 10.1038/s41594-021-00699-y

Extended Data Fig. 3. Liposome recordings of purified A3/B3 channel proteins.

Extended Data Fig. 3

a, Single-channel currents of purified A3/B3 activated by 10 μM cGMP. A total of 29 patches were recorded. Of them, 17 showed no cGMP activity and 12 showed cGMP-activated activity. b, Continuous trace of A3/B3 single-channel current showing inhibition by L-cis-diltiazem (DTZ). c, Amplitude histogram of cGMP-induced currents at + 80 mV from one patch. d, Single-channel conductance of cGMP-induced currents. Data were presented as mean±SD. e, Continuous trace of the same patch as in (b) showing a preference of cGMP over cAMP. f, Single-channel open probability of currents evoked by 10 μM cGMP or 10 μM cAMP. Data were presented as mean±SD.