Table 1.
Cryo-EM data collection, refinement and validation statistics.
| CNGA3/CNGB3 apo (EMD-24468, PDB 7RHS) | |
|---|---|
|
| |
| Data collection and processing | |
| Magnification | 105,000 × |
| Voltage (kV) | 300 |
| Electron exposure (e−/Å2) | 57.63 |
| Defocus range (μm) | −0.6 to −2.2 |
| Pixel size (Å) | 0.8247 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 3,982,214 |
| Final particle images (no.) | 205,492 |
| Map resolution (Å) | 2.93 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 2.7 to 3.1 |
| Refinement | |
| Initial model used | De novo structure |
| Model resolution (Å) | 3.06 |
| FSC threshold | 0.143 |
| Map sharpening B factor (Å2) | −103 |
| Model composition | |
| Non-hydrogen atoms | 14,767 |
| Protein residues | 1,804 |
| Sugar moieties | 3 |
| Ion (Na+) | 1 |
| B factors (Å2) | |
| Protein | 85.39 |
| Sugar moieties | 89.59 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.006 |
| Bond angles (°) | 0.945 |
| Validation | |
| MolProbity score | 1.28 |
| Clashscore | 3.47 |
| Poor rotamers (%) | 0.06 |
| Ramachandran plot | |
| Favored (%) | 97.22 |
| Allowed (%) | 2.78 |
| Disallowed (%) | 0 |