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. Author manuscript; available in PMC: 2023 Jan 19.
Published in final edited form as: Neuron. 2021 Nov 8;110(2):209–220.e6. doi: 10.1016/j.neuron.2021.10.019

Figure 5. Chemogenetic activation of sympathetic nerve activity in the DRG enhances the incidence of CFEs and spontaneous Pain.

Figure 5.

(A) Diagram showing the mating strategy and intrathecal injection with AAV9.CAG.GCaMP6s in Phox2bCre; GqDREADD mice.

(B) Diagram showing the experimental procedure. The details are the same as described in Figure 4B.

(C) An example of increased cluster firing after local addition of CNO on DRG. Left is quiescent; Middle is an example of cluster firing before addition of CNO. There are 2 clusters (orange and purple circles); Right is after addition of CNO, and there are 3 clusters (orange, purple and red circles).

(D) Representative traces of neurons in the orange cluster which are numbered in (c) before and after administration of CNO.

(E-G) The total number of clusters (E), frequency of cluster firing (F), and total number of cluster firing neurons (G) significantly increased after addition of CNO but not vehicle to the DRG.

(H) Spontaneous pain scores significantly increased after i.t. administration of CNO to SNI mice (Day 20) with cluster firing, but not after administration of vehicle.

Scale bar, 100 μm. Each pair of open circles (before and after) represents an individual mouse. Data are represented as mean ± SEM. *p < 0.05; **p < 0.01; ****p < 0.0001; n.s., not significant;(E) to (H) by two-way repeated measures ANOVA with Sidak’s posttest.