Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-tyrosine hydroxylase antibody | Pel-Freeze | Cat# P40101 RRID: AB_2617184 |
| Anti-CD31 antibody | BD-Biosciences | Cat# 550274 RRID: AB_393571 |
| Goat anti-Rat Secondary Antibody, Alexa Fluor 647 | Invitrogen/Thermo Fisher | Cat# A21247 RRID: AB_141778 |
| Bacterial and virus strains | ||
| AAV9-CAG-GCaMP6s | Addgene (Chen, et al.,2013) | pAAV.CAG.GCaMP6s.WPRE.SV40 Catalog # 100844-AAV9 |
| Custom AAV9-CAG-NE2h | Vigene Biosciences | N/A |
| Custom AAV9-CAG-DA4.4 | Vigene Biosciences | N/A |
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| 6-Hydroxydopamine hydrochloride | Sigma-Aldrich | Cat# H4381-100MG |
| Phentolamine hydrochloride | Sigma-Aldrich | Cat# P7547-100MG |
| (±)-Propranolol hydrochloride | Sigma-Aldrich | Cat# P0884-1G |
| Doxazosin mesylate | Sigma-Aldrich | Cat# D9815-50MG |
| Lidocaine HCl | Vetone | Cat# 510213 |
| Atipamezole | Sigma-Aldrich | Cat# A9611 |
| Carbenoxolone disodium salt | Sigma-Aldrich | Cat# C4790-1G |
| Clozapine N-oxide | Sigma-Aldrich | Cat# C0832-5MG |
| Dopamine hydrochloride | Sigma-Aldrich | Cat# H8502-25G |
| Noradrenaline bitartrate | Tocris | Cat# 5169 |
| Cholera toxin subunit B, Alexa Fluor™ 555 Conjugate | Invitrogen | Cat# C22843 |
| Urea | Sigma-Aldrich | Cat# U5378-1KG |
| N,N,N',N'-Tetrakis(2-Hydroxypropyl)ethylenediamin | Sigma-Aldrich | Cat# 122262-1L |
| Triton X-100 | Sigma-Aldrich | Cat# T9284-100ML |
| Adenosine 5′-triphosphate (ATP) disodium | Sigma-Aldrich | Cat# A1852-30MG |
| Paraformaldehyde Solution, 4% in PBS | Thermo Scientific | Cat# AAJ19943K2 |
| DMEM | Gibco | Cat# 11995065 |
| Fetal calf serum | Gibco | Cat# 26140079 |
| Critical commercial assays | ||
| RNeasy Plus Micro Kit | Qiagen | Cat# 74034 |
| RNase-free DNase | Qiagen | Cat# 79256 |
| iScript cDNA synthesis kit | Bio-Rad | Cat# 1708891BUN |
| Deposited data | ||
| Experimental models: Cell lines | ||
| B16F10 melanoma cells | ATCC | Cat# ATCC® CRL-6475™ |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6 mice | Jackson Laboratories | Cat# JAX:000664 RRID: IMSR_JAX:000664 |
| Mouse: Phox2b-Cre | Jackson Laboratories | Cat# JAX: 016223 RRID:IMSR_JAX:016223 |
| Mouse: GFAP-Cre | Jackson Laboratories | Cat# JAX: 012886 RRID:IMSR_JAX:012886 |
| Mouse: Ai14 strain B6.Rosa26-stop (flox)-tdTomato | Jackson Laboratories | Cat# JAX: 007914 RRID:IMSR_JAX:007914 |
| Mouse: Rosa26-LSL-hM3Dq-DREADD | Jackson Laboratories | Cat# JAX: 026220 RRID:IMSR_JAX:026220 |
| Mouse: Rosa26-LSL-hM4Di-DREADD | Jackson Laboratories | Cat# JAX: 026219 RRID:IMSR_JAX:026219 |
| Mouse: Pirt-Cre | This lab (Kim et al., 2016) | N/A |
| Mouse: Pirt-GCaMP3 (GFP) | This lab (Kim et al., 2008) | N/A |
| Mouse: Rosa26-LSL-GCaMP6s | Bergles Lab at JHU | N/A |
| Oligonucleotides | ||
| Assay ID:Mm00442668_m1 ; Gene Symbol: Adra1a | Applied Biosystems | Cat# 4331182 |
| Assay ID:Mm00431685_m1 ; Gene Symbol:Adra1b | Applied Biosystems | Cat# 4331182 |
| Assay ID:Mm01328600_m1 ; Gene Symbol:Adra1d | Applied Biosystems | Cat# 4331182 |
| Assay ID:Mm07295458_s1; Gene Symbol:Adra2a | Applied Biosystems | Cat# 4331182 |
| Assay ID:Mm00477390_s1 Gene Symbol:Adra2b | Applied Biosystems | Cat# 4331182 |
| Assay ID:Mm00431686_s1 ; Gene Symbol:Adra2c | Applied Biosystems | Cat# 4331182 |
| Assay ID:Mm00431701_s1; Gene Symbol:Adrb1 | Applied Biosystems | Cat# 4331182 |
| Assay ID:Mm02524224_s1 Gene Symbol:Adrb2 | Applied Biosystems | Cat# 4331182 |
| Assay ID:Mm02601819_g1 Gene Symbol:Adrb3 | Applied Biosystems | Cat# 4331182 |
| Assay ID:Mm01205647_g1 Gene Symbol:Actb | Applied Biosystems | Cat# 4331182 |
| RecombinantDNA | ||
| Software and algorithms | ||
| FIJI | NIH | RRID: SCR_003070 |
| Prism 8, 9 | GraphPad Software | N/A |
| Cellsens Dimension imaging software | Olympus | N/A |
| Other | ||
| Confocal microscope | Leica | TCS LSI |
| Fluorescent microscope | Olympus | BX63 |
| StepOnePlus real-time PCR system | Applied Biosystems | 4376598 |