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. 2022 Jan 20;79(2):84. doi: 10.1007/s00018-021-04125-w

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© The Author(s) 2022

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Fig. 3 — Effect of LPS administration on GFP + EV release to the circulation. a Schematic protocol of Tamoxifen induction and LPS injection of mice. b, c Flow cytometry analysis. Calibration beads were used (0.2–1 µm). d The gating strategy for mEVs and the 1 μm counting beads. e As a negative control, PFP-derived mEVs were analyzed from C57Bl6 mice. f Cardiomyocyte-derived EVs as GFP⁺ events. mEVs secreted by all other cells were Tomato⁺. g A 95 ± 1% reduction of the event number after exposure to 0.1% Triton. h, i Events within the mEV gate were then analyzed, and GFP + events with/without LPS injection are shown on (h). i demonstrates Tomato⁺ events with/without LPS injection. The figure shows the results of three independent experiments (n = 9). The figure represents data from individual mEV samples (mice) as well the means ± SD (*P < 0.05, T-test). j Fold increase upon LPS treatment, n = 6 ns = nonsignificant (T-test). k The pie diagram represents the percentages of green fluorescent mEVs in control and LPS treated animals (n = 9 in each group)