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. 2021 Sep 2;16(2):555–568. doi: 10.1038/s41396-021-01097-4

Fig. 3. VirClust hierarchical clustering of the new dsDNA flavophages and their relatives (reduced dataset), based on intergenomic distances calculated using the protein cluster content.

Fig. 3

For an extended tree, including the larger ICTV dataset, see SI file 3. 1. Hierarchical clustering tree. Two support values, selective inference (si, [99]) and approximately unbiased (au, [100]), are indicated at branching points (si/au) only for the major clades (see SI file 1 Figs. 6 and 7 for all support values). The tree was cut into smaller viral genome clusters (VGCs) using a 0.9 distance threshold. Each VGC containing our flavophages was proposed here as a new family. Exceptions were made for the “Aggregaviridae” and “Forsetiviridae”, for which only part of the VGC were included in the families, to exclude some genomes with lower support values. Each VGC is framed in a rectangle in 2 and 3. 2. Silhouette width, measures how related is a virus with other viruses in the same VGCs. Similarity to other VGCs is indicated by values closer to -1 (red). Similarity to viruses in the same VGC is indicated by values closer to 1 (green). 3. Distribution of the protein clusters (PCs) in the viral genomes. 4. Genome length (bps). 5. Fraction of proteins shared with other viruses (dark gray), based on protein assignment to PCs. 6. Virus names, with flavophages isolated in this study marked in red. 7. Genus assignment. Gray bars indicate already defined genera by the ICTV with the following names from top to bottom: Silviavirus, Labanvirus, Unahavirus, Pippivirus, Lillamyvirus, Muminvirus, Lillamyvirus, Helsingorvirus, and Incheonvirus. 8. Habitat. 9. Host association, as determined by: 9a) BlastN; 9b) CRISPR spacers; 9c) WIsH with host database GEM. 10. Life style genes. 11. TEM images of the new flavophages, uranyl acetate negative staining. Scale bar in each TEM image has 50 nm 12. Newly proposed families. Full name of environmental phages in “Winoviridae” and unclassified: AP013511.1_Uncultured_Mediterranean_phage_uvMED,_group_G21,_isolate__uvMED-CGR-C117A-MedDCM-OCT-S32-C49 and, AP013358.1_Uncultured_Mediterranean_phage_uvMED,_group_G1,_isolate__uvMED-CGR-U-MedDCM-OCT-S27-C45, respectively.