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. 2021 Aug 31;16(2):606–610. doi: 10.1038/s41396-021-01098-3

Fig. 2. Visualization of Loki- and Heimdallarchaeota cells in Aarhus Bay sediments by CARD-FISH.

Fig. 2

Probe names and the dyes are indicated for each panel. Representative cell morphotypes were imaged in a super-resolution structured illumination microscope (SR-SIM; panels (A), (B), (D), (E)) and confocal laser scanning microscope (CLSM; panels (C) and (F)). For SR-SIM images, single slices from the center of the focal plane are shown. For CLSM images, three-dimensional (3D) surface reconstructions are depicted. All z-stack images taken in CLSM are included in Supplementary Fig. 2. 360° rotation of 3D reconstructed images are also provided in Supplementary Video. Negative and positive controls are shown in Supplementary Fig. 1 together with large ovoid and filamentous false-positive signals. Images are representative of dual labeled Lokiarchaeota (n = 72) and Heimdallarchaeota (n = 70) cells in five individual experiments using two different sediment cores taken from the same sampling site. The scale bar is 1 µm.