Table 1.
Content of phenolic compounds detected using HPLC in the extracts of H. rhamnoides L. leaves and twigs.
| No. according to HPLC RT order | Phenolic compoud | Synonymus | Quantification wavelengths A 235; 280; 325; 375 nm Em 420 nm (Ex 270 nm) |
Content [mg/g] in dry matter of extract | |
|---|---|---|---|---|---|
| Leaf | Twig | ||||
| 1 | Gallic acid | (3.4.5-Trihydroxybenzoic acid) | 280 | 1.633 ± 0.149 | 2.160 ± 0.208 |
| 2 | p-Benzoquinone | (Quinone) | 235 | 2.059 ± 0.189 | 1.484 ± 0.143 |
| 3 | α-resorcylic acid | (3.5-Dihydroxybenzoic acid) | 420 | 2.302 ± 0.211 | 12.149 ± 1.191 |
| 4 | Pyrocatechol | (1.2-Dihydroxybenzene. Catechol) | 280 | 0.248 ± 0.233 | 0.326 ± 0.041 |
| 5 | Protocatechuic acid | (3.4-Dihydroxybenzoic acid) | 420 | 0.419 ± 0.041 | 32.832 ± 3.161 |
| 6 | Neochlorogenic acid | (trans-5-O-Caffeoylquinic acid) | 325 | 0.182 ± 0.021 | 0.830 ± 0.087 |
| 7 | (−)-epigallocatechin | (monomeric flavan-3-ol) | 235 | 6.035 ± 0.592 | 6.765 ± 0.651 |
| 8 | (+)-Catechin | (flavan-3-ol) (monomeric flavan-3-ol) | 235 | 2.052 ± 0.186 | 14.962 ± 1.444 |
| 9 | 4-hydroxybenzoic acid | – | 235 | 3.923 ± 0.228 | 1.948 ± 0.198 |
| 10 | Procyanidin B2 | (pentahydroxyflavane); (cis.cis″-4.8″-Bi(3.3′.4′.5.7-pentahydroxyflavane) (Polymeric flavan-3-ol) | 280 | 13.933 ± 1.276 | 15.549 ± 1.498 |
| 11 | Gentisic acid | (2.5-Dihydroxybenzoic acid) | 325 | 1.380 ± 0.162 | 0.334 ± 0.006 |
| 12 | 4-Hydroxybenzaldehyde | – | 280 | 0.171 ± 0.016 | 0.185 ± 0.0187 |
| 13 | Chlorogenic acid | (trans-3-O-Caffeoylquinic acid) | 325 | 0.144 ± 0.013 | 0.058 ± 0.006 |
| 14 | Vanilic acid | (4-Hydroxy-3-methoxybenzoic acid) | 420 | 0.142 ± 0.015 | 0.328 ± 0.031 |
| 15 | β-resorcylic acid | (2.4-Dihydroxybenzoic acid) | 420 | 0.278 ± 0.025 | 0.889 ± 0.091 |
| 16 | Caffeic acid | (trans-3.4-Dihydroxycinnamic acid) | 325 | 0.132 ± 0.014 | 0.045 ± 0.005 |
| 17 | (−)-epicatechin | (monomeric flavan-3-ol) ((−)-cis-3.3′.4′.5.7-Pentahydroxyflavane) | 235 | 2.353 ± 0.225 | 1.440 ± 0.123 |
| 18 | Syringic acid | (4-Hydroxy-3.5-dimethoxybenzoic acid) | 420 | 0.758 ± 0.066 | 0.902 ± 0.047 |
| 19 | 1.3-Dicaffeoylquinic acid | 325 | 0.182 ± 0.021 | 0.062 ± 0.006 | |
| 20 | Cyanidin | (3.3′.4.5.7-Pentahydroxyflavone) (3.3′.4.5.7-Pentahydroxyflavylium chloride) | 280 | 1.665 ± 0.153 | 0.974 ± 0.098 |
| 21 | Syringaldehyde | (4-Hyroksy-3.5-dimethoxybenzaldehyde) | 280 | 0.228 ± 0.024 | 0.969 ± 0.102 |
| 22 | p-Coumaric acid | (trans-4-Hydroxycinnamic acid) | 325 | 0.677 ± 0.069 | 0.488 ± 0.047 |
| 23 | Ferulic acid | (4-Hydroxy-3-methoxy-cinnamic acid) | 420 | 0.137 ± 0.014 | 0.286 ± 0.006 |
| 24 | Coumarin | (1.2-Benzopyrone) | 280 | 0.311 ± 0.029 | 0.237 ± 0.023 |
| 25 | Sinapic acid | (4-Hydroxy-3.5-dimethoxy-cinnamic acid) | 420 | 0.129 ± 0.004 | 0.552 ± 0.055 |
| 26 | trans-3-Hydroxycinnamic acid | (m-Coumaric acid) | 280 | 0.491 ± 0.051 | 0.122 ± 0.011 |
| 27 | Luteolin | 7-O-β-d-glucoside | 325 | 2.018 ± 0.089 | 0.583 ± 0.061 |
| 28 | Rutin | (quercetin-3-O-rutinoside) | 375 | 7.791 ± 0.731 | 0.649 ± 0.051 |
| 29 | Ellagic acid | (4.4′.5.5′.6.6′-Hexahydroxydiphenic acid 2.6.2′.6′-dilactone) | 235 | 4.444 ± 0.416 | 0.456 ± 0.047 |
| 30 | Hesperidin | (Hesperetin-7-rutinoside) | 280 | 4.949 ± 0.311 | 0.610 ± 0.062 |
| 32 | o-Coumaric acid | (trans-2-Hydroxycinnamic acid) | 420 | 0.289 ± 0.029 | 0 |
| 31 | Rosmarinic acid | (R,E)-3-(3,4-dihydroxyphenyl)-2-((3-(3,4-dihydroxyphenyl)acryloyl)oxy)propanoic acid | 420 | 11.642 ± 1.065 | 1.452 ± 0.014 |
| 33 | Salicylic acid | (2-Hydroxybenzoic acid) | 420 | 0.701 ± 0.067 | 0.961 ± 0.098 |
| 34 | Myricetin | (flavonol) (3.3′.4′.5.5′.7-Hexahydroxyflavone) | 375 | 0.720 ± 0.075 | 0.413 ± 0.042 |
| 35 | Quercetin | (flavonol) (3.3′.4′.5.7-Pentahydroxyflavone) | 375 | 0.149 ± 0.011 | 0.049 ± 0.005 |
| 36 | trans-Cinnamic acid | 280 | 0.136 ± 0.014 | 0.094 ± 0.009 | |
| 37 | Naringenin | (4′.5.7-Trihydroxyflavanone) | 280 | 0.019 ± 0.002 | 0.236 ± 0.032 |
| 38 | Luteolin | (3′.4′.5.7-Tetrahydroxyflavone) | 325 | 2.772 ± 0.266 | 0.787 ± 0.077 |
| 39 | Kaempferol | (3.4′.5.7-Tetrahydroxyflavone) | 375 | 0.193 ± 0.015 | 0.026 ± 0.003 |
| 40 | 3-Hydroxyflavone | Flavonol | 325 | 0.131 ± 0.016 | 0.125 ± 0.012 |
Values are means ± SD (n = 3).