Table 1.
Timeline for experimental protocols.
| Timeline | Animal procedures |
|---|---|
| Day 0 | NeuRet-MSCV-Cre lentiviral vectors injection into the right cuneiform nucleus (CnF) |
| 3–7 days after 1st viral injection | AAV-EF1α-DIO-hChR2 (H134R)-FP injection into the right deeper layers of superior colliculus (dSC) |
| 4–8 weeks after 2nd viral injection | Bilateral lesion of amygdala by ibotenic acid injection |
| 3–6 days after amygdala lesion | Optic fiber implantation on the right dSC |
| 5–31 days after optic fiber implantation | Behavior testing induced by optogenetic stimulation of the right dSC (> 6 weeks after NeuRet-MSCV-Cre lentiviral vectors injection and > 1 week after bilateral lesion of amygdala) |