Online-only Table 1.

Metrics of protein quantification via serial SDS-PAGE.

Gel 1	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	4851	1	25	50	0.5
CTRL	3121	0.64	16	32.2	0.5
ERL	2289	0.47	12	23.6	0.5
BOS	2099	0.43	11	21.6	0.5
VSN	2003	0.41	10	20.6	0.5
NIL	3498	0.72	18	36.1	0.5
Gel 2	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	8468	1	25	50	0.5
CTRL	3622	0.43	11	21.4	0.5
AXI	5688	0.67	17	33.6	0.5
PAZ	5929	0.7	18	35	0.5
RUX	6428	0.76	19	38	0.5
AFA	7152	0.84	21	42.2	0.5
Gel 3	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	8468	1	25	50	0.5
CTRL	3622	0.43	11	21.4	0.5
AXI	5688	0.67	17	33.6	0.5
PAZ	5929	0.7	18	35	0.5
RUX	6428	0.76	19	38	0.5
AFA	7152	0.84	21	42.2	0.5
Gel 4	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	9789	1	25	50	0.5
NIL	5914	0.6	15	30.2	0.5
AXI	7570	0.77	19	38.7	0.5
PAZ	7507	0.77	19	38.3	0.5
RUX	7172	0.73	18	36.6	0.5
AFA	6802	0.69	17	34.7	0.5
Gel 5	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	8720	1	25	50	0.5
ERL	5983	0.69	17	34.3	0.5
SOR	6080	0.7	18	34.9	0.5
BOS	6559	0.75	19	37.6	0.5
VSN	6436	0.74	19	36.9	0.5
Gel 6	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	2337	1	25	50	0.5
NIL	1988	0.85	21	42.5	0.5
AXI	1660	0.71	18	35.5	0.5
PAZ	1844	0.79	20	39.5	0.5
RUX	1757	0.75	19	37.6	0.5
AFA	1895	0.81	20	40.5	0.5
Gel 7	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	3409	1	25	50	0.5
CTRL	2767	0.81	20	40.6	0.5
SOR	2006	0.59	15	29.4	0.5
Gel 8	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	2439	1	25	50	0.5
CTRL	1786	0.73	18	36.6	0.5
BOS	1878	0.77	19	38.5	0.5
VAN	2200	0.9	23	45.1	0.5
NIL	1953	0.8	20	40	0.5
AXI	2096	0.86	22	43	0.5
Gel 9	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	2708	1	25	50	0.5
CTRL	2282	0.84	21	42.1	0.5
RUX	2144	0.79	20	39.6	0.5
AFA	2199	0.81	20	40.6	0.5
REG	2965	1.09	27	54.7	0.5
REG	2070	0.76	19	38.2	0.5
Gel 10	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	2869	1	25	50	0.5
CTRL	2236	0.78	20	39	0.5
SUN	1880	0.66	17	32.8	0.5
CTRL	2262	0.79	20	39.4	0.5
SUN	2240	0.78	20	39	0.5
Gel 11	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	2317	1	25	50	0.5
CTRL	1760	0.76	19	38	0.5
PAZ	1321	0.57	14	28.5	0.5
REG	1575	0.68	17	34	0.5
SUN	1888	0.81	20	40.7	0.5
Gel 12	Density	Ratio	Protein Amount (μg)	Resuspend Volume(ul)	Conc.(ug/ul)
HeLa	1444	1	25	50	0.5
CTRL	1121	0.78	20	38.8	0.5
REG	995	0.69	17	34.5	0.5
CTRL	1062	0.74	19	36.8	0.5
SUN	1023	0.71	18	35.4	0.5