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. 2021 Aug 21;16(2):331–338. doi: 10.1038/s41396-021-01088-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A The current state of research focused on inoculum establishment. Without quantitative prioritization, potential barriers like the illustrated examples are investigated randomly. B Path forward to improve establishment success. Prioritization of barriers includes quantifying their relative impact in order to create decision trees that can simultaneously rank barriers and summarize the potential impact of overcoming each barrier in a multi-factor solution path. Values in parentheses on the decision tree illustrate an example of percentage impact of a barrier on inoculum establishment, guiding microbiome engineering investments.