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. 2022 Jan 20;12:1097. doi: 10.1038/s41598-022-04998-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental setup and stimulus design. (a) Behavioral setup. Computer-generated visual and auditory stimuli were delivered through a video projector and a water-proof speaker, respectively. A clear acetate sheet delimited the experimental arena. An LED was turned on synchronously with auditory stimulus onset while a high speed camera recorded fish behavior, visual stimuli and the state of the LED for the duration of each trial. (b) The visual loom expanded from a subtended angle of 2° to 100° in 5.3 s (L/V = 0.192). When visual and auditory stimuli were combined, the auditory stimulus onset preceded the end of visual expansion by 160 ms. (c) Six intensities of visual stimuli (Michelson Index, MI) and 6 intensities of auditory stimuli (amplitude in dB, re 1 µPa) were combined into 36 different multisensory stimuli, thus creating a 2D space of multisensory intensities.