Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 20;12:1048. doi: 10.1038/s41598-022-05140-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Analysis of down- and upregulated pathways in 2-mo Pcyt2^+/− liver. (A) Downregulated genes from 2-mo Pcyt2^+/− liver were analyzed with Enrichr (https://maayanlab.cloud/Enrichr/)^33–35 (n = 3). (A-a) Pathway analysis (MGI Mammalian Phenotypes Level 4–2019) established the most enriched terms include hepatic steatosis and increased circulating ammonia. (A-b) The Gene Onthology (GO) analysis (GO Biological Processes 2018) further shows that the most significantly downregulated were processes of fatty acid oxidation and nitrogen degradation. The most frequently downregulated GO genes from phospholipids and fatty acid metabolism and nitrogen (urea cycle, arginine) are indicated in the clustergram. (B) Upregulated genes from 2-mo Pcyt2^+/− liver were analyzed with Enrich (n = 3). (B-a) The cluster analysis for the Elsevier Pathway Collection identified as elevated the Igf2- and Ang4/Ang2-Foxo1 pathways and ion/amino acid transport. (B-b) GO Biological Processes 2018 of the most upregulated genes identified as the most important the processes linked to Ang2/Ang4 functions in regulation of angiogenesis and Egfr signaling. (C) Go:Human Phenotype establish a gene/disease network for young Pcyt2^+/− with significant risk for development of maternal- and type 2- diabetes and related pathologies. (D) Immunoblot analysis of lipid pathways in fasted and fed Pcyt2^+/+ and Pcyt2^+/− (n = 4). Band intensities were measured using ImageJ. Data are presented as mean ± SD. *p < 0.05; **p < 0.01.

Analysis of down- and upregulated pathways in 2-mo Pcyt2^+/− liver. (A) Downregulated genes from 2-mo Pcyt2^+/− liver were analyzed with Enrichr (https://maayanlab.cloud/Enrichr/)^33–35 (n = 3). (A-a) Pathway analysis (MGI Mammalian Phenotypes Level 4–2019) established the most enriched terms include hepatic steatosis and increased circulating ammonia. (A-b) The Gene Onthology (GO) analysis (GO Biological Processes 2018) further shows that the most significantly downregulated were processes of fatty acid oxidation and nitrogen degradation. The most frequently downregulated GO genes from phospholipids and fatty acid metabolism and nitrogen (urea cycle, arginine) are indicated in the clustergram. (B) Upregulated genes from 2-mo Pcyt2^+/− liver were analyzed with Enrich (n = 3). (B-a) The cluster analysis for the Elsevier Pathway Collection identified as elevated the Igf2- and Ang4/Ang2-Foxo1 pathways and ion/amino acid transport. (B-b) GO Biological Processes 2018 of the most upregulated genes identified as the most important the processes linked to Ang2/Ang4 functions in regulation of angiogenesis and Egfr signaling. (C) Go:Human Phenotype establish a gene/disease network for young Pcyt2^+/− with significant risk for development of maternal- and type 2- diabetes and related pathologies. (D) Immunoblot analysis of lipid pathways in fasted and fed Pcyt2^+/+ and Pcyt2^+/− (n = 4). Band intensities were measured using ImageJ. Data are presented as mean ± SD. *p < 0.05; **p < 0.01.