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. 2022 Jan 20;12:1048. doi: 10.1038/s41598-022-05140-y

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PEA stimulates phospholipid and triglyceride metabolic genes. (A) Immunoblot analysis of liver Pcyt2α and Ctl1 in 8-mo Pcyt2^+/+, Pcyt2^+/− and Pcyt2^+/− + PEA mice treated for 12 weeks (n = 3–4). mRNA analysis of (B) phospholipid and (C) lipid metabolism genes in Pcyt2^+/+, Pcyt2^+/− and Pcyt2^+/− + PEA mice. GAPDH used as loading control. Band intensities were measured using ImageJ. Membranes were cut prior to antibody hybridization to allow for the probing of multiple targets on one membrane. Data are presented as mean ± SD. *p < 0.05, **p < 0.01 relative to Pcyt2^+/+; ^#p < 0.05, ^##p < 0.01 relative to Pcyt2^+/−.