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. 2022 Jan 20;5:78. doi: 10.1038/s42003-022-03021-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative time-lapse images of HeLa/GFP-LacI cells transfected with pLacO-pEF1α-RFP. DNA was stained with Hoechst 33342. Immediately after transfection, acquisition of time-lapse images at 10-min intervals was started. Time 0 represents the leftmost image (680 min after the start of image acquisition). DNA (magenta) and GFP-LacI (white) in the merged images. Bar, 10 μm. b HeLa/GFP-LacI cells transfected with pLacO-pEF1α-RFP, fixed, and stained for emerin as indicated in Fig. 1b. A single, representative prometaphase cell is shown. The upper and lower rows show the same cell at focal planes 1.5 μm apart. Arrowhead indicate puncta with emerin signals. DAPI (DNA, blue), GFP-LacI (green), and emerin (red) are shown in the merged image. Bars, 10 μm. b′ Enlarged image of the lower panel of GFP-LacI in Fig. 2b. Arrows indicate small puncta. c A metaphase cell is shown in the same manner as in b. Bar, 10 μm. d Super-resolution images of GFP-LacI and chromosomes in a metaphase cell. HeLa/GFP-LacI cells were transfected with pLacO-pEF1α-RFP. After transfection, cells were fixed with a fixative as described in Methods. Super-resolution images were obtained using LSM880 Airyscan microscopy. White and magenta represent small puncta of transfected DNA (GFP-LacI) and chromosomes (DAPI), respectively. Bar, 10 μm. e Diagrams of transfected DNA in interphase, prometaphase, and metaphase cells.