TABLE 2.

Degree of reproducibility of results of four AST methodologies with regard to 30 consecutive MICs for reference strains C. parapsilosis ATCC 22019 and C. krusei ATCC 6258

Medium	Inoculum (CFU/ml)	Method of endpoint determination	Time of incubation (h)	ICCa with:
				AMB	5FC	FLC	ITC	KTC	VRC
RPMI–2%G	105	Visual inspection	24	0.843	0.956	0.791	0.789	0.825	0.777
			48	0.856	0.942	0.722	0.783	0.812	0.799
		Spec-50%	24	0.854	0.978	0.932	0.962	0.971	0.934
			48	0.823	0.921	0.908	0.927	0.937	0.936
		Spec-80%	24	0.935	0.943	0.912	0.897	0.907	0.886
			48	0.921	0.956	0.899	0.904	0.911	0.901
		Spec-95%	24	0.965
			48	0.964
RPMI–2%G	104	Visual inspection	24	0.813	0.914	0.786	0.756	0.863	0.797
			48	0.824	0.931	0.798	0.765	0.821	0.804
		Spec-50%	24	0.845	0.932	0.911	0.941	0.962	0.908
			48	0.865	0.941	0.903	0.916	0.924	0.912
		Spec-80%	24	0.921	0.916	0.895	0.868	0.894	0.870
			48	0.919	0.932	0.891	0.857	0.904	0.868
		Spec-95%	24	0.943
			48	0.931
RPMI–2%G	103	Visual inspection	24	0.813	0.907	0.782	0.743	0.829	0.799
			48	0.819	0.906	0.784	0.725	0.822	0.812
		Spec-50%	24	0.853	0.896	0.853	0.842	0.895	0.892
			48	0.855	0.885	0.849	0.834	0.894	0.867
		Spec-80%	24	0.901	0.893	0.842	0.840	0.881	0.867
			48	0.888	0.871	0.856	0.829	0.874	0.866
		Spec-95%	24	0.929
			48	0.921
RPMI	103	Visual inspection	24	0.845	0.723	0.654	0.765	0.798	0.723
			48	0.898	0.912	0.867	0.781	0.825	0.804
		Spec-50%	24	0.734	0.723	0.712	0.698	0.687	0.714
			48	0.742	0.889	0.863	0.821	0.886	0.843
		Spec-80%	24	0.765	0.678	0.703	0.843	0.812	0.823
			48	0.845	0.829	0.851	0.820	0.839	0.818
		Spec-95%	24	0.870
			48	0.871

^aData are expressed over a maximum value of 1.