TABLE 4.
AST results by Candida spp. obtained with RPMI–2%G and an inoculum of 105 CFU/ml after 24 and 48 h of incubation and comparison with those obtained by the NCCLS reference procedure
| Species (no. of isolates) | Antifungal agent | Range of MIC (μg/ml)
|
||
|---|---|---|---|---|
| RPMI–2%G and inoculum of 105 CFU/ml after incubation fora:
|
NCCLS reference method | |||
| 24 h | 48 h | |||
| C. albicans (10)b | AMB | 0.50–1.00 | 0.50–2.00 | 0.50–2.00 |
| 5FC | 0.12–0.25 | 0.12–1.00 | 0.12–0.50 | |
| FLC | 0.12–0.50 | 0.12–0.50 | 0.25–0.50 | |
| ITC | 0.12–0.25 | 0.12–0.25 | 0.12–0.50 | |
| KTC | ≤0.01–0.03 | ≤0.01–0.03 | ≤0.01–0.03 | |
| VRC | ≤0.01–0.03 | ≤0.01–0.06 | ≤0.01–0.06 | |
| C. tropicalis (10) | AMB | 0.50–1.00 | 0.50–2.00 | 1.00–2.00 |
| 5FC | 0.12–1.00 | 0.12–2.00 | 0.12–2.00 | |
| FLC | 0.25–8.00 | 0.25–8.00 | 0.25–8.00 | |
| ITC | 0.06–0.50 | 0.12–0.50 | 0.12–1.00 | |
| KTC | ≤0.01–0.06 | ≤0.01–0.12 | ≤0.01–0.25 | |
| VRC | ≤0.01–0.03 | ≤0.01–0.03 | ≤0.01–0.03 | |
| C. parapsilosis (10) | AMB | 0.50–2.00 | 0.50–2.00 | 0.50–2.00 |
| 5FC | 0.12–0.50 | 0.12–0.50 | 0.12–0.25 | |
| FLC | 0.25–2.00 | 0.25–4.00 | 0.25–2.00 | |
| ITC | 0.12–0.25 | 0.12–0.25 | 0.12–0.50 | |
| KTC | ≤0.01–0.03 | ≤0.01–0.06 | ≤0.01–0.06 | |
| VRC | ≤0.01–0.06 | ≤0.01–0.12 | ≤0.01–0.12 | |
| C. glabrata (10) | AMB | 0.25–2.00 | 1.00–2.00 | 1.00–2.00 |
| 5FC | 0.12–0.50 | 0.12–0.50 | 0.12–0.50 | |
| FLC | 4.00–16.0 | 8.00–64.0 | 4.00–64.0 | |
| ITC | 1.00–4.00 | 1.00–16.0 | 1.00–16.0 | |
| KTC | 0.06–0.25 | 0.25–0.50 | 0.12–0.50 | |
| VRC | 0.12–4.00 | 0.25–8.00 | 0.12–8.00 | |
| C. krusei (10) | AMB | 0.12–2.00 | 0.25–2.00 | 0.06–1.00 |
| 5FC | 1.00–8.00 | 1.00–8.00 | 0.50–16.0 | |
| FLC | 16.0–64.0 | 32.0–128.0 | 16.0–128.0 | |
| ITC | 0.25–1.00 | 0.25–1.00 | 0.12–2.00 | |
| KTC | 0.06–0.50 | 0.12–1.00 | 0.12–0.50 | |
| VRC | 0.12–0.50 | 0.25–1.00 | 0.12–1.00 | |
| C. lusitaniae (10) | AMB | 0.12–2.00 | 0.12–2.00 | 0.12–1.00 |
| 5FC | 0.12–1.00 | 0.12–4.00 | 0.12–4.00 | |
| FLC | 0.25–0.50 | 0.25–0.50 | 0.25–1.00 | |
| ITC | ≤0.01–0.12 | ≤0.01–0.25 | ≤0.01–0.25 | |
| KTC | ≤0.01–0.03 | ≤0.01–0.03 | ≤0.01–0.03 | |
| VRC | ≤0.01–0.03 | ≤0.01–0.03 | ≤0.01–0.03 | |
| C. parapsilosis ATCC 22019c | AMB | 0.25–2.00 | 1.00–2.00 | 0.50–2.00 |
| 5FC | 0.12–0.50 | 0.12–0.25 | 0.12–0.5 | |
| FLC | 1.00–4.00 | 2.00–4.00 | 1.00–4.00 | |
| ITC | 0.12–0.25 | 0.12–0.50 | 0.25–0.50 | |
| KTC | ≤0.01–0.06 | 0.03–0.12 | 0.03–0.12 | |
| VRC | ≤0.01–0.12 | 0.03–0.25 | 0.03–0.12 | |
| C. krusei ATCC 6258c | AMB | 0.25–2.00 | 1.00–2.00 | 0.50–2.00 |
| 5FC | 4.00–8.00 | 4.00–8.00 | 8.00–16.0 | |
| FLC | 16.0–32.0 | 32.0–64.0 | 16.0–64.0 | |
| ITC | 0.12–0.5 | 0.12–0.50 | 0.25–1.00 | |
| KTC | 0.06–0.50 | 0.12–0.50 | 0.12–0.50 | |
| VRC | 0.12–0.50 | 0.12–0.50 | 0.25–0.50 | |
a
MICs represented were obtained with the Spec-95% for AMB and the Spec-50% for 5FC, FLC, ITC, KTC, and VRC.
b
All procedures were repeated on two different days.
c
Data from 30 consecutive determinations on different days.