Figure 1.

Mice with primary immunodeficiencies and normal C57BL/6 (B6) mice were vaccinated twice with Δcps1 avirulent vaccine and challenged IN with 50-100 arthroconidia of virulent C. posadasii Silveira. (A) Lung fungal burdens quantitated 14 days post-infection were significantly reduced in Stat4 KO, mut-Stat3, Dectin-1 KO and Ifngr KO mice compared to unvaccinated B6. Vaccinated Rag-1 KO mice, deficient in T- and B-cells, had no reduction in fungal burdens. (B) Dissemination was prevented in most vaccinated PID mice and statistically significantly reduced in all strains while universally present in unvaccinated B6 mice. (Statistical analysis – Kruskal- Wallis).