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. 2022 Jan 7;12:758862. doi: 10.3389/fphys.2021.758862

FIGURE 2.

FIGURE 2

Expression of TmIKKε during the developmental stages and in different tissues of T. molitor. (A) qRT-PCR was used to measure the expression of TmIKKε mRNA in the different developmental stages of the insect. YL, early instar larvae; LL, late-instar larvae; PP, pre-pupa; P1–P7, 1- to 7-day-old pupa; A1–A5, 1- to 5-day-old adult. (B) Expression of TmIKKε mRNA in tissues of T. molitor LL as analyzed using real-time polymerase chain reaction (qRT-PCR). (C) Expression of TmIKKε messenger RNA (mRNA) in the tissues of T. molitor adults as analyzed using qRT-PCR. GT, Gut; IT, integument; FB, fat body; MT, Malpighian tubules; HC, hemocytes. T. molitor ribosomal protein 27a (TmL27a) was used as an internal control to normalize the concentration of templates between samples. Vertical bars represent mean ± SE (n = 20).