Table 1. Metabolic and kidney function measurements in diabetic and control animals receiving either MCC950 treatment or vehicle only.
Short-term study (10 weeks of diabetes) | Long-term study (18 weeks of diabetes) | |||||||
---|---|---|---|---|---|---|---|---|
Vehicle | MCC950 in week 5–10 | Vehicle | MCC950 in week 9–18 | |||||
Control | Diabetes | Control | Diabetes | Control | Diabetes | Control | Diabetes | |
Body weight (g) | 30.8 ± 0.5 | 25.8 ± 1.1*** | 29.5 ± 0.5 | 25.9 ± 0.9** | 31.7 ± 0.5 | 24.6 ± 1.4*** | 30.7 ± 1.0 | 26.3 ± 1.4** |
Right kidney/BW (mg/g) | 6.17 ± 0.15 | 7.3 ± 0.40** | 6.49 ± 0.17 | 6.91 ± 0.29 | 6.7 ± 0.16 | 8.44 ± 0.44*** | 6.30 ± 0.13 | 7.95 ± 0.47*** |
Left kidney/BW (mg/g) | 6.31 ± 0.16 | 7.20 ± 0.42* | 6.31 ± 0.14 | 6.51 ± 0.20 | 6.8 ± 0.11 | 8.21 ± 0.39** | 6.22 ± 0.13 | 8.09 ± 0.61*** |
P-glucose (mmol/l) | 10.3 ± 0.4 | 25.7 ± 2.1*** | 10.2 ± 0.6 | 23.6 ± 1.2*** | 10.1 ± 0.4 | 30.6 ± 1.8*** | 11.6 ± 0.6 | 29.3 ± 1.7*** |
HbA1c (%) | 4.3 ± 0.2 | 9.1 ± 0.6*** | 4.0 ± 0.1 | 8.1 ± 0.3*** | 4.1 ± 0.1 | 9.7 ± 1.1** | 4.4 ± 0.2 | 9.3 ± 1.0** |
Systolic BP (mmHg) | 99 ± 4.6 | 101 ± 4.6 | 88 ± 6.7 | 92 ± 6.8 | 98 ± 3.8 | 100 ± 3.2 | 103.8 ± 6.2 | 98.5 ± 5.9 |
Food intake (g) | 2.9 ± 0.2 | 3.7 ± 0.2 | 2.8 ± 0.2 | 4.0 ± 0.4** | 2.6 ± 0.15 | 5.1 ± 0.3*** | 2.7 ± 0.3 | 4.3 ± 0.5*** |
Water intake (ml) | 2.8 ± 0.6 | 10.1 ± 1.6*** | 2.1 ± 0.3 | 9.5 ± 2.9*** | 1.9 ± 0.3 | 17.0 ± 2.5*** | 1.8 ± 0.2 | 15.3 ± 3.2*** |
Urine output (ml) | 1.06 ± 0.17 | 9.9 ± 1.5*** | 1.1 ± 0.14 | 8.7 ± 2.9*** | 0.85 ± 0.17 | 15.56 ± 2.13*** | 0.65 ± 0.14 | 13.69 ± 3.02*** |
Cystatin C (ng/ml) | 300 ± 34 | 204 ± 19** | 293 ± 24 | 224 ± 13* | 230 ± 11 | 190 ± 12* | 233 ± 13 | 176 ± 14** |
ACR (mg/g) | 45.1 ± 3.6 | 126.3 ± 17.5** | 50.4 ± 3.1 | 104.4 ± 22.2* | 30.2 ± 4.9 | 78.2 ± 13.8* | 41.1 ± 6.5 | 160.5 ± 40.3**# |
Data are presented as mean ± SEM. Abbreviations: ACR, urinary albumin-to-creatinine ratio; BP, blood pressure; BW; body weight; P; plasma. n=7–11 per group. Asterisks represent P-values for the comparisons of diabetes vs. control and diabetes+MCC950 vs. control+MCC950, respectively. *<0.05, **<0.01, *** <0.001. # represents P=0.06 for the comparison of diabetes+MCC950 vs untreated diabetes.