Fig. 5.

Challenged tumor inhibition and enhanced anti-tumor immune memory via activating of Chl. coupled PDT in vivo. a, Experimental design to evaluate the tumor re-challenge inhibition and anti-tumor immune memory effect on Balb/c mice (4T1 tumor cells). b, Tumor volume of the local 4T1 tumors post various treatments. c, Local tumor complete responses rate post different treatments (CR: complete responses). d, Re-challenged tumor volume of the 4T1 tumors post various treatments after tumor challenge. e, Re-challenged tumor complete responses rate post different treatments (CR: complete responses). f, Body weight of the mice post various treatments. g, Flow cytometry analysis quantification of the CD44^hiCD62L^low T_em cells in spleen post the four treatments (gated on CD3⁺CD8⁺ cells). h, Relative LDH in the supernatant after co-incubation of spleen cells and 4T1 tumor cells. i, IFN-γ concentration in the supernatant after co-incubation of spleen cells and 4T1 tumor antigen. Each experimental group included n ≥ 3. Data are representative or pooled and are expressed as Mean ± SE. Asterisks indicate statistically significant differences as analyzed by One-Way ANOVA (∗∗∗ p < 0.001).