Fig. 2.

SDS PAGE with reduced and non-reduced purified protein samples. Lanes 1 and 4: molecular weight ladder; Lanes 2 and 3: non-reduced and DTT-reduced protein samples, respectively. The non-reduced sample appeared as a single band at the 14 kDa region, while the reduced sample appeared as two bands at the 9 and 5 kDa regions resulting from disruption of di-S bridges in the protein.