Figure 2.

Live cation imaging using open Drosophila antenna preparations. (A) Schematic of the cation imaging workflow. D. melanogaster antennae are first excised, then deposited into Drosophila Ringer solution and held in place with two-component glue prior to bisection using micro-scissors. Every antenna is bisected to better reveal the funiculus interior for live cell imaging with fluorescence microscopy. (B) A non-bisected antennal whole mount unsuitable for imaging cells, for comparison. Scale bar: 20 μm. (C) Exemplar fluorescence micrographs of tormogen support cells, thecogen support cells, and the Orco-positive subset of olfactory sensory neurons, prior to cation imaging (targeted using nompA-, ASE5, and Orco-GAL4 drivers, respectively). Cell type-specific fluorescent reporter expression of Ca²⁺ indicator GCaMP6f and K⁺ indicator GINKO1 are shown by column. Scale bars: 20 μm.