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GFP-fused CYVMV V1 (CYVMV-KoV1-GFP) and V2 (CYVMV-KoV2-GFP) deletion constructs were created for individual N. benthamiana leaf infiltrations. GFP expression evidenced through epifluorescence (A,B) and confocal imaging (C,D). Deletion of either V1 or V2 restricted GFP signals within the infiltration sites.
