Fig. 6.

Endometrial regeneration, collagen remodeling, and fertility restoration induced by the AB-laden HA hydrogel in a rat intrauterine adhesion model. (Print in colour) a) Schematic of the in vivo animal experiment procedure. b) Representative images of uteri treated for 60 d, examined using hematoxylin and eosin (H&E) staining and Masson's trichrome staining. The insets outlined by black dashed boxes are shown in the lower row at a higher magnification. c) Quantification of endometrial thickness at days 30 and 60 post-surgery using H&E staining (n = 10). d) Quantification of endometrial glands at days 30 and 60 post-surgery using H&E staining (n = 10). e) Masson's trichrome staining scores for uteri at days 30 and 60 after treatment (n = 10). f) Violin plots showing the number of embryos per uterine horn following different treatments (n = 33 in the NR and ABs groups, n = 29 in the HA and HA/ABs groups). g) Rat embryos at gestational day (GD) 19 and neonate from the HA/ABs group. h) Representative images of placenta from the HA/ABs group obtained on GD 19 examined using H&E staining. The insets outlined by black dashed boxes are shown at a higher magnification. *p < 0.05 and **p < 0.01. NR, natural repair after synechiae division; ABs, injection of an AB solution only after synechiae division; HA, injection of HA only after synechiae division; HA/ABs, injection of the AB-laden HA hydrogel after synechiae division.