FIGURE 1.

miR‐204 inversely regulates the stretch response of H9C2 cells. (A and B) The effect of 20% stretch (after 24 h) on the expression of (A) miR‐204 (mature and precursor) and (B) load‐response genes (nppa, nppb and β‐mhc) in H9C2 cells (n = 7). (C) The effect of miR‐204 mimic (miR‐204 M, 100 nM) on the expression of load‐response genes in H9C2 cells during stretching (n = 6). (D) The effect of miR‐204 inhibitor (miR‐204 I, 100 nM) on the expression of load‐response genes in H9C2 cells during stretching (n = 9). Data are shown as the mean, and error bars represent SEM. *p < .05 versus indicated group. SC, scrambled control; nppa, natriuretic peptide a; nppb, natriuretic peptide b; β‐mhc, beta‐myosin heavy chain