Fig. 8.

BI853520 attenuates orthotopic tumor growth through PI3K/AKT/mTOR signaling pathway in vivo. A, B The mean mouse weights following treatment with BI853520, paclitaxel monotherapy and combination treatment. C, D Protein expression levels of p-FAK (Y397) were examined in mice tumors following treatment with BI853520, paclitaxel monotherapy and combination treatment using Western blot analysis. E–G Protein expression levels of p-AKT/AKT and p-mTOR(S2448)/mTOR were measured in mice tumors following treatment with BI853520, paclitaxel monotherapy and combination treatment using Western blot analysis. H, I p-AKT and p-mTOR(S2448) in mice tumors were examined using imunofluorescence staining assay. The data were presented as mean ± SD of three independent experiments. **P < 0.01