Fig. 4.

Overexpressed E2F7 in PCa attenuated the suppressive role of miR-26a. A E2F7 mRNA levels in the indicated tissues samples were detected using RT-qPCR. The representative IHC staining of VEGFA in PCa tissues and adjacent normal tissues. Scale bar, 50 um. B The protein levels of E2F7 in normal and PCa cells were compared by Western blot. C Spearman correlation test showed the inverse correlation between miR-26a and E2F7 in PCa. D We transfected PCa cells with the indicated vectors and detected E2F7 expression. E, F Reintroduction of E2F7 significantly reversed the suppressed proliferation by miR-26a. G The endogenous level of E2F7 was knocked out by Caspir-cas9, and the silencing efficiency was confirmed via Western blotting. H, I Silencing of E2F7 expression further inhibited PCa cell proliferation by miR-26a overexpression. *P < 0.05, **P < 0.01, and ***P < 0.001