Table 4.
The different larvae groups used to evaluate the effectiveness of phage therapy for the treatment of challenged larvae.
| Groups 1 | Description of Larvae Groups 2 |
|---|---|
| Controls | |
| C-1 | Healthy larvae |
| C-2 | Administered PBS |
| C-3 | Administered the 2-phage cocktail SPFM10-SPFM14 |
| C-4 | Administered the 3-phage cocktail SPFM4-SPFM10-SPFM19 |
| C-5 | Administered the 4-phage cocktail SPFM2-SPFM10-SPFM14-SPFM19 |
| C-6 | Challenged with Salmonella |
| Prophylactic phage treatments | |
| P-2 | Administered 2-phage cocktail SPFM10-SPFM14 one hour prior to challenge with Salmonella |
| P-3 | Administered 3-phage cocktail SPFM4-SPFM10-SPFM19 one hour prior to challenge with Salmonella |
| P-4 | Administered 4-phage cocktail SPFM2-SPFM10-SPFM14-SPFM19 one hour prior to challenge with Salmonella |
| Co-infection studies | |
| CoI-2 | Administered 2-phage cocktail SPFM10-SPFM14 simultaneously with Salmonella |
| CoI-3 | Administered 3-phage cocktail SPFM4-SPFM10-SPFM19 simultaneously with Salmonella |
| CoI-4 | Administered 4-phage cocktail SPFM2-SPFM10-SPFM14-SPFM19 simultaneously with Salmonella |
1 In each group 60 larvae were used for the study. Every 24 h 20 larvae were checked for survival and culled to enumerate PFU and CFU counts. 2 Phages, challenge with MDR S. Typhimurium S01160-12 strain and PBS were administered orally.