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. 2022 Jan 6;15(1):72. doi: 10.3390/ph15010072

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Confocal microscopy for Na⁺/K⁺-ATPase α1 cell surface expression in cultured HCECs. HCECs seeded on coverslips and incubated with ST or OU with/without EEDK for 24 h and then stained with Na⁺/K⁺-ATPase antibody (green fluorescence, membranous) and DAPI (blue). (A) Un-treated HCECs (CNT), (B) HCECs treated with 2 µg/mL EEDK, (C) cells treated with 5 nM ST, (D) HCECs undergone co-treatment of ST and EEDK, (E) HCECs with 50 nM OU, (F) cells co-treated with OU and EEDK, and (G,H) graphical representation of ST and OU group respectively. Results are expressed as the mean ± SEM of three independent experiments. ** p < 0.01, *** p < 0.001 (one-way ANOVA with Dunnett’s multiple comparison).