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Schematic for the development of ASFV-G-∆A859L. The transfer vector contains the p72 promoter and a mCherry cassette; the gene positions are indicated. The homologous arms were designed to have flanking ends to both sides of the deletion/insertion cassette. The nucleotide positions of the area that was deleted in the ASFV-G genome are indicated by the dashed lines. The resulting ASFV-G-∆A859L virus with the cassette inserted is shown on the bottom.
