Table 1.
Bacterial strains, plasmids, and bacteriophages used in this study.
| Strains/Plasmid/Bacteriophage | Relevant Genotype or Annotation | Source |
|---|---|---|
| E. coli | ||
| DH5α | F−, gyrA96(Nalr), recA1, relA1, endA1, thi-1, hsdR17(rk−, mk+), glnV44, deoR, Δ(lacZYA-argF)U169, [φ80dΔ(lacZ)M15], supE44 | SCPM-O * |
| Y. pestis | ||
| 231 | wild type strain; universally virulent (LD50 for mice ≤ 10 CFU, for guinea pigs ≤ 10 CFU); Pgm+, pMT1, pPCP1, pCD− | SCPM-O [23] |
| KM260(12) | avirulent derivative of 231; Pgm+, pMT1−, pPCP1−, pCD− | SCPM-O |
| KM260(12)ΔlpxM | ΔlpxM derivative of KM260(12) | SCPM-O |
| KM260(12)ΔlpxM/pEYR’ | KM260(12)ΔlpxM containing pEYR’ | This study |
| KM260(12)ΔlpxM/pEYR’-E | KM260(12)ΔlpxM containing pEYR’-E | This study |
| KM260(12)ΔlpxM/pEYR’-E-Y-K | KM260(12)ΔlpxM containing pEYR’-E-Y-K | This study |
| KM260(12)ΔlpxM/pEYR’-Y-K | KM260(12)ΔlpxM containing pEYR’-Y-K | This study |
| KM260(12)ΔlpxM/pEYR’-E-S-R-Rz | KM260(12)ΔlpxM containing pEYR’-E-S-R-Rz | This study |
| KM260(12)ΔlpxM/pEYR’-S-R-Rz | KM260(12)ΔlpxM containing pEYR’-S-R-Rz | This study |
| Plasmid | ||
| pACYC184 | Source of p15A ori and cat gene | [24] |
| pBAD/myc-HisA | Source of rrnB transcription terminator | Invitrogen |
| pET32b (+) | Source of multiple cloning site | Novagene |
| pEYR’ | Expression vector, phage Lambda modified right promoter (pR’) (Cmr) | SCPM-O |
| pEYR’-E | Lysis plasmid, pEYR’-lysis E (Cmr) | SCPM-O |
| pEYR’-E-Y-K | Lysis plasmid, pEYR’-lysis E, Y, K (Cmr) | SCPM-O |
| pEYR’-Y-K | Lysis plasmid, pEYR’-lysis Y, K (Cmr) | SCPM-O |
| pEYR’-E-S-R-Rz | Lysis plasmid, pEYR’-lysis E, S, R, Rz (Cmr) | SCPM-O |
| pEYR’-S-R-Rz | Lysis plasmid, pEYR’-lysis S, R, Rz (Cmr) | SCPM-O |
| Bacteriophage | ||
| λCE6 | Source of pR promoter, holin (S) and endolysins (R-Rz) genes (cI857Sam7) | Thermo Scientific |
| φX174 | Source of E protein gene | Thermo Scientific |
| L-413C | Source of holin (Y) and endolysin (K) genes | Russian Research Anti-Plague Institute Microbe |
* The State Collection of Pathogenic Microbes and Cell Cultures on the base of the State Research Center for Applied Microbiology and Biotechnology (“SCPM-Obolensk”).