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. 2022 Jan 1;10(1):67. doi: 10.3390/vaccines10010067

Figure 2.

Figure 2

Construction of Salmonella Typhimurium expressing engineered spike protein as an antigen. (a) Schematic representation of the pKU-Ag.e.spike vector expressing engineered spike protein. (b) Expression of engineered spike protein in genetically engineered S. Typhimurium was measured by Western blot. (c) RAW 264.7 cells were infected with the indicated S. Typhimurium at a multiplicity of infection of 500:1 for 1 h (for invasion assay). The invasion rate of murine RAW 264.7 cells by the unmodified S. Typhimurium was set to 100%, and the relative internalization levels were normalized against those of unmodified S. Typhimurium. The data represent the mean ± standard error of three independent experiments. (d) Stability of pKU-Ag.e.spike in S. Typhimurium without antibiotic selection. Data represent the mean ± SD of a representative experiment.