TABLE 1.
Primers used
| Primera | Sequence (5′–3′) | Expected size of product (bp) |
|---|---|---|
| ermB1 | AAA(C/T)TGATTTTT(A/T)GTAAA | 530 |
| ermB2 | AGGTAAAGGGCATTT | |
| ermA1 | AGGTTATAATGAAACAGA | 208 |
| ermA2 | GCATGACATAAACCTTCA | |
| mefA1 | CTATGACAGCCTCAATGCG | 1,400 |
| mefA2 | ACCGATTCTATCAGCAAAG | |
| tetM(f)1 | ATAGACACGCCAGGACATAT | 1,116 |
| tetM(f)2 | GTTTATCACGGAAGTGCAA | 686 |
| tetM(r)3 | GGAGCCCAGAAAGGATTCGG | |
| speA1 | CTTAAGAACCAAGAGATGGC | 200 |
| speA2 | ATAGGCTTTGGATACCATCG | |
| speB1 | TTCTAGGATACTCTACCAGC | 300 |
| speB2 | ATTTGAGCAGTTGCAGTAGC | |
| speC1 | CATCTATGGAGGAATTACGC | 246 |
| speC2 | TGTGCCAATTTCGATTCTGC | |
| MF1 | ATAAGGAGCATAAAAATGGCT | |
| MR1 | AGCTTAGTTTTCTTCTTTGCG | 500–1,100b |
| MF2 | TATT(C/G)GCTTAGAAAATTAA | |
| MR2 | GCAAGTTCTTCAGCTTGTTT |
a
f, forward primer; r, reverse primer.
b
Depending on the emm gene.