Figure 3.

(A) A microplate assay for measuring OD 600 nm of HSA (5 mg/mL) colored by value (>0.5 means aggregation). (B) SAXS-based screening of HSA (5 mg/mL) colored by the value of the radius of gyration R_g. (C) Changes in R_g value were obtained for HSA at extreme formulations with 40 °C. (D) Mean radii distribution of HSA at extreme formulations with 40 °C before and after 48 h measured by DLS. (E) SDS-polyacrylamide gel electrophoresis (SDS-PAGE) of HSA incubated at extreme formulations with 40 °C before and after 48 h.