Table 6.
List of forward and reverse primers used for the bacterial quantification by QPCR. Bp: base pairs; Tm: melting temperature.
| Target Bacterial Group | Reverse Primer (5′-3′) Forward Primer (5′-3′) |
Amplicon Length (bp) | Tm (°C) | References |
|---|---|---|---|---|
| Total bacteria | F: GTGCCAGCMGCCGCGGTAA R: GACTACCAGGGTATCTAAT |
291 | 64.2 52.4 |
[75] |
| Lactobacillus spp. | F: AGCAGTAGGGAATCTTCCA R: CACCGCTACACATGGAG |
341 | 54.5 55.2 |
[76] |
| Bifidobacterium spp. | F: GCGTGCTTAACACATGCAAGTC R: CACCCGTTTCCAGGAGCTATT |
125 | 60.3 59.8 |
[77] |
| Enterobacteriaceae | F: ATGTTACAACCAAAGCGTACA R: TTACCYTGACGCTTAACTGC |
185 | 54.0 56.3 |
[78] |