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. 2022 Jan 12;14(1):129. doi: 10.3390/v14010129

Figure 2.

Figure 2

EnvCT W790A virus does not perturb HIV-1 Env function and viral cell–cell spread. (A) Alanine substitution of the tryptophan at HIV-1 envelope position 790 does not affect the ability of infected Jurkat cells to form conjugates with uninfected target cells. (B,C) EnvCT W790A-infected cells also form synapses to the same extent as WT HIV-1 do and result in no defect in both intracellular and surface localization of Env. (D) Quantification of Env (gp120) incorporation into virions produced in Jurkat cells, normalised to Gag (p24) density. (E) Quantification of surface Env MFI in infected Jurkat cells measured by flow cytometry analysis of cells stained with bNab PGT151. (F) Cell surface W790A Env staining (solid black line, unfilled) and WT virus (dark grey, filled) measured by flow cytometry staining using bNab PGT151 (top) or 2G12 (bottom). Uninfected control is shown as a light grey-filled histogram. (G,H) Budding of W790A virions from infected cells (G), and the relative infectivity (H) of W790A virus is equivalent to that of WT. (I) Spreading infection of W790A virus between Jurkat T cells over time (measured by flow cytometry analysis of the percentage Gag+ cells) is equivalent to that of WT virus, but is defective for W757A virus.