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. 2021 Dec 27;14(1):52. doi: 10.3390/pharmaceutics14010052

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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In vitro cell cellular uptake analysis by (A) CLSM after HepG2 cells incubated with (a) Free DOX, (b) (Dex)-βCD-(PCL)₁₄@DOX-SPIO, (c) (Dex-SS)-βCD-(PCL)₁₄@DOX-SPIO, (d) (FA-Dex)-βCD-(PCL)₁₄@DOX-SPIO and (e) (FA-Dex-SS)-βCD-(PCL)₁₄@DOX-SPIO at 5 μg/mL DOX for 4 h (scale bar = 100 μm). (B) DOX fluorescence intensity of HepG2 cells incubated with free DOX and DOX-SPIO-loaded polymeric nanoparticles at 5 μg/mL of DOX for 4 h measured by flow cytometry.