Figure 3.

(a-i) The single-cell printer consists of a dispenser chip (1) mounted to an aluminum case with a piezo stack actuator, a substrate, or microplate (2) for single-cell printing, an illumination system (3), a CCD camera (4) for cell detection, and a reservoir (5) for cell suspension loading. (a-ii) The size of the ROI was determined by the volume of the printed droplet. (a-iii) A single HeLa cell array was printed on a substrate. Reproduced with permission from [40]. (b) Schematic illustration of the real-time cellular recognition-based single-cell printing system using the microfluidic dispenser chip and droplet generation. Reproduced with permission from [41].