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. 2021 Dec 21;14(1):5. doi: 10.3390/v14010005

Figure 2.

Figure 2

Plasma from individuals infected by SARS-CoV-2 or BNT162b2 vaccinated donors recognized Spike at the surface of human pAECs infected with authentic viruses. pAECs (from two different subjects) were either (A) mock-infected or (B,D,F,G) infected with authentic SARS-CoV-2 D614G or (C,E,F,G) the Alpha variants. In (A–C,F), plasma recovered from eight convalescent individuals whose symptoms had begun 6 or 11 weeks previously was used to stain infected pAECs. Data shown consist of a representative staining of infected pAECs with the plasma from one donor (A–C), and the median fluorescence intensities (MFI) obtained on N+ cells with plasma from all convalescent donors normalized to the CV3-25 antibody (F). In (D,E,G), plasma from nine individuals who were SARS-CoV-2 naïve and vaccinated obtained before vaccination (V0–open circle), 3 weeks (V1–black triangle) and 12 weeks (V2–green triangle) after first mRNA dose, or 3 weeks after the second dose (V3–red triangle) (administered with a 16-week interval between doses) were used to detect infected pAECs. Data shown consist of a representative staining of pAECs infected with SARS-CoV-2 D614G (D) or Alpha variant (E) with plasma from one donor, and the MFI obtained on N+ cells with plasma from all vaccinated individuals normalized to the CV3-25 antibody (G). Statistical significance was tested using RM one-way ANOVA (F, Alpha variant in G), Frieman test (D614G in G), and paired t test (between D614G and Alpha variant in F and G) (* p < 0.05; ** p < 0.01, *** p < 0.001, and **** p < 0.0001).