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. 2022 Jan 13;23(2):861. doi: 10.3390/ijms23020861

Figure 1.

Figure 1

Identification of coe2 mutant. (A) Phenotype of 5-day-old seedlings of coe2 and WT on 1/2 MS plates. (B) Heatmap of the expression of genes of light-harvesting proteins in cotyledons of seedlings of coe2 and WT under normal growth conditions. (C) Statistical analysis of the root length in 5-day-old seedlings of WT and coe2. Three biological replications were used. The student’s t-test of coe2 versus WT, ** p < 0.001. (D) The SNP-index of coe2. SNPs between coe2 and the reference genome were analyzed by Bulked Segregant Analysis. (E) The candidate mutation of coe2 was identified by DNA sequencing. (F) The mutation of coe2 (blue star) and coe2-1 (triangle, SALK_047882) are marked on the gene map. (G) Relative expression of COE2 in coe2, coe2-1, and WT was determined by qPCR. Three biological replicates were used. The student’s t-test of coe2 or COM. Versus WT, * p < 0.05%, ** p < 0.01%, *** p < 0.001.