Table 1.
Selected parameters of study methods from experimental studies.
| Parameters | Study A | Study B | Study C | Study D |
|---|---|---|---|---|
| Virus | ||||
| Name a | LY (MF318451) | ISU27734 (MK058528) | N/A | JX (MK656956) |
| Origin | China, 2015 | USA, 2018 | N/A | China, 2018 |
| Farms of origin | ||||
| Clinical signs/lesions | PDNS-like lesions | Lymphocytic myocarditis | N/A | Diarrhea |
| Affected pigs | Piglet | Piglet (8 days) | Fetus and suckling piglet | Suckling and weaned pigs |
| Inocula | ||||
| Infectious material | Virus isolate (infective clone) | Virus isolate | Tissue homogenate | Intestinal content |
| Route and titer b | IN: 2 × 106.53 TCID50 | IN: 6.6 × 1010 gc IM: 6.6 × 1010 gc |
IN: 2.04 × 1011 gc IM: 6.38 × 1012 gc (twice, 7 days apart) |
Oral: 3.0 × 106.5 gc |
| Inoculated pigs | ||||
| Breed | Duroc × Large White | N/A | N/A | N/A |
| Age | 4 and 8 weeks old | 6 weeks old | 5 weeks old | 3 weeks old |
| Farrowing status | Conventional | CD/CD | CD/CD | N/A |
| PCV3 detection | ||||
| Virus titer | qPCR (ORF2) | qPCR (ORF2) | qPCR (ORF2) | None |
| Tropism | IHC (PCV3 antigen) | ISH (ORF1 mRNA) | ISH (ORF1 mRNA) | None |
| Antibody detection | ||||
| Target | Capsid | Capsid | Capsid | None |
| Isotype | N/A | IgM and IgG | IgM and IgG | None |
| Duration of studies | 28 days | 28 days | 42 days | 7 days |
Study A [18]; Study B [17]; Study C [19]; Study D [13]; N/A, data not available; gc, genomic copy; IN, intranasal; IM, intramuscular; IHC, immunohistochemistry; ISH, in situ hybridization; CD/CD, cesarian-derived and colostrum-deprived; TCID50, median tissue culture infectious dose; ORF, open reading frame. a GenBank accession number is in parenthesis. b Virus titers were shown as total titers (calculated from ‘volume x concentration’ reported in the original papers).