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. 2021 Dec 28;8(1):20. doi: 10.3390/jof8010020

Figure 4.

Figure 4

Human Beta Defensin-1 (hBD1) expression in cells of cervicovaginal lavage. (A) Immunofluorescence of cervicovaginal lavage cytospin samples from Control individuals and patients with AVVC or RVVC. Upper panel (2nd Ab control), cytospin of cervicovaginal lavage from patients with RVVC. Cell nuclei were stained with DAPI (blue), and hBD1 expression is shown in green. Images are shown at ×630 magnification. (B) Fluorescence semiquantification was performed using the ImageJ software. The assay could not be performed in all the patients of each cohort due to sample limitations. Mean ± SEM. Control n = 4, AVVC n = 6, RVVC n = 5. * p < 0.05. (C) hBD1 mRNA expression by real-time PCR in cervicovaginal lavage cells from Control individuals and patients with AVVC or RVVC. The assay could not be performed in all the patients of each cohort due to sample limitations. Mean ± SEM. Control n = 14, AVVC n = 5, RVVC n = 15. ** p < 0.01, *** p < 0.001. ANOVA test.