TABLE 2.

Comparison of three blood sample preparation methods for PCR diagnosis of VL in dogs

Dog code no.	No. of positive PCRs/no. of PCRs performed (intensity of banding pattern in ethidium bromide-stained gels) for sample preparation methoda
	WB-GE-S	BC-PK-ΦC	BC-K
14	0/4	4/4 (++++)	4/4 (++++)
15	4/4 (+++)	4/4 (++++)	4/4 (++++)
16	4/4 (++)	4/4 (++++)	4/4 (++++)
17	2/4 (++)	4/4 (++++)	4/4 (+++)
20	2/4 (++)	4/4 (++++)	4/4 (++++)
21	4/4 (+++)	4/4 (++++)	4/4 (++++)
24	0/4	4/4 (++++)	4/4 (++++)

^a++ to ++++, least intense to most intense, respectively.