Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Representative scheme of the direct RNA seq. protocol (SQK-RNA002, Nanopore Technologies). dsRNA-DNA hybrids are synthesized during the library preparation. Next, the adapters needed for sequencing through nanopores are added at the 3′ poly A end, with the motor protein bound in 3′ to 5′ direction. Thereby, only the RNA strand will be sequenced, whereas the cDNA strand will be excluded.
