Table 1.
Minimum inhibitory concentrations (MIC) of Checacin1 and Checacin1 fragments determined for different microbes (Ec: Escherichia coli, Pa: Pseudomonas aeruginosa, Ms: Mycobacterium smegmatis, Sa: Staphylococcus aureus, Af: Aspergillus flavus, Ca: Candida albicans). CAMH-II: cation-adjusted Mueller Hinton II medium; CAMH-C: CAMH-II with 44 mM sodium bicarbonate; TEM-1: TEM-1 beta-lactamase expressing strain; MRSA: methicillin-resistant Staphylococcus aureus; BTG: BacTiter-Glo™-assay; MTT: microtiter turbidity assay.
| Compounds | MIC (µM) | ||||||
|---|---|---|---|---|---|---|---|
| Ec | Pa | Ms | Sa | Af | Ca | ||
| ATCC 35218 | ATCC27853 | ATCC607 | ATCC33592 | ATCC9170 | FH2173 | ||
| TEM-1 | TEM-1 | MRSA | |||||
| CAMHII | CAMH-C | CAMHII | CAMHII | CAMHII | CAMHII | CAMHII | |
| Checacin1 | 1.6–0.8 | 1.6 | 12.5 | 25 | 1.6 | 50 | 6.25 |
| Checacin11−11 | >50 | >50 | >50 | >50 | >50 | >50 | >50 |
| Checacin112−25 | >50 | >50 | >50 | >50 | >50 | >50 | >50 |
| Checacin11−21 | >50 | >50 | >50 | >50 | 12.5 | >50 | >50 |
| Rifampicin | 4.9 | 19.4 | 38.9 | 38.9–19.4 | >77.8 | NA | NA |
| Tetracycline | 4.5 | 36–18 | >144.1 | 0.14 | >72 | NA | NA |
| Gentamycin */Isoniazid ’’ | 2.1–1 | 1–0.5 | 1 | 29.2–14.6 ’’ | 0.5–0.26 | NA | NA |
| Tebuconazole | NA | NA | NA | NA | NA | 0.19 | 0.19–0.09 |
| Amphotericin B | NA | NA | NA | NA | NA | 1.1 | 8.6–4.3 |
| Nystatin | NA | NA | NA | NA | NA | 1.1 | 8.6 |
| readout | MTT | MTT | MTT | BTG | MTT | BTG | BTG |
* Calculated for gentamycin C1 (25876-10-2) as gentamycin (1405-41-0) is a substance mixture. ’’ Mycobacterium smegmatis was tested against isoniazid instead of gentamycin.